Phenol solution, ACS – CAS 108-95-2

A tris-equilibrated phenol solution is a clear liquid capped with a layer of Tris buffer.
Phenol solutions can be used for a wide variety of molecular biology applications. Organic compounds like phenols have an aromatic carbocyclic ring with a hydroxyl group attached directly to it. The common name for monohydroxybenzene is phenol.
Phenol solution has been used:
# as a component of the digestion reaction mix to prepare RNase-free plasmids for in vitro translation in a rabbit reticulocyte lysate ;
# for extraction of DNA from Arabidopsis thaliana Rosette leaves ;
# in the extraction of DNA from blood on preservation paper by standard phenol-chloroform protocol ;
# as a component of extraction buffer to extract RNA from fruit pulp ;
# for the extraction of protein from roots ;
# for the isolation of fecal DNA ;
# for the extraction of caged RNA .
The phenol-chloroform extraction method is widely employed to extract nucleic acids; even though phenol does not completely inhibit RNase activity, its unique characteristic makes it beneficial for the isolation of nucleic acids when used in combination with chloroform and alcohol