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Follistatin human (HPLC), 20 μg [* micrograms] CAS 7601-54-9 - https://www.eurchem.net

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technical file
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Follistatin human (HPLC) , sterilized
FS, activins-binding protein
100% made in European Union *(according to USP, EUP, Echa-Reach)
Tech. / Lab. / pharmaceutical / bio-research / cell culture
ES5403A
7601-54-9
231-509-8
human
expressed in E. coli
cell culture
suitable
suitable for molecular biology
>99.99%
lyophilized (from 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.)
20 μg (* micrograms)
maintain refrigerated at 2-8°C for up to 6 months; for long term storage store at -20°C
Follistatin is expressed in the pituitary, ovaries, decidual cells of the endometrium, and in some other tissues; the gene is mapped to human chromosome 5q11.2.
Recombinant human Follistatin is a 31.5kDa protein containing 288 amino acids.
Its primary structure contains three cysteine-rich domains (called FS domains), each followed by a protease-inhibitory kazal domain.
Application:
Follistatin human has been used to suppress myostatin signaling; It has also been used as an inhibitor of activin A.
Biochem/physiol Actions:
Follistatin is a multifunctional secreted protein that binds to ligands of the TGF (transforming growth factor)-β family and regulates their activity by inhibiting their access to signaling receptors ; It was
originally discovered as an activin antagonist whose activity suppresses expression and secretion of the pituitary hormone FSH (follicle-stimulating hormone) ; In addition to being a natural antagonist, follistatin can inhibit the activity of other TGF (transforming growth factor)-β ligands including BMP (bone morphogenetic protein)-2,-4,-6,-7, Myostatin, GDF-11 (growth differentiation factor 11), and TGF-b1; in gonads, it is responsible for oocyte competence and embryo development. In the pituitary gland, it causes cyclic changes in FSH production in the estrous cycle. Follistatin also participates in bone metabolism. It controls inflammation, fibrosis, and tissue repair ; levels of plasma follistatin increase in polycystic ovary syndrome and type 2 diabetes.
Follistatin might also be involved in tumor progression, including apoptosis, angiogenesis and metastasis
Preparation:
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
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for research use only ; not for use in diagnostic procedures
Special for chemical transport (according to IATA / ADR / ECHA-REACH rules / int.l laws) .
According to U.S./E.U. Pharmacopoeia, Echa-Reach, UNI EN ISO 14001, G.M.P., MSDS, etc...

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